Figure 2.
Characterization of proviral clones in vitro. 293 T cells (1.5 × 105) were cotransfected with 2 μg wtHTLV-1, HTLV-1ΔHBZ, HTLV-1HBZΔLZ proviral clones, or negative control DNA along with 0.1 μg LTR-1–Luc and 0.01 μg TK-Renilla. All transfections were performed in triplicate and normalized to TK-Renilla to control for transfection efficiency. Cell lysates or supernatants were harvested 48 hours after transfection. Histograms present the average values from 3 independent experiments; error bars denote (SD). (A) Measure of Tax activity presented as relative luciferase units. (B) Measure of p19 Gag in the cellular supernatants.
