The D′-D3 domain of VWF is sufficient to recruit the P-selectin lumenal domain. HEK293 cells were nucleofected with various VWF constructs (A) and either a soluble lumenal domain of P-selectin (A-B) or a mutated P-selectin (A,C). VWF is stained red, and P-selectin is stained green (note that for D1-D3-GFP, the red and green layers have been interchanged to be consistent with the rest of the figure). Panels on the right are enlargements of the regions boxed with dashed lines on the left. The enlarged top panel is VWF, and the bottom panel is P-selectin. Note that the P-selectin pattern is very different when coexpressed with the propeptide because it is not recruited to the VWF-positive puncta.