Dual immunohistochemistry staining of bone marrow trephines with primary antibodies to CD138 and MAGE/NY-ESO. (A,C) Negative stains of bone marrow from patients 4 and 22, respectively. (B,D) Cytoplasmic and nuclear expression of MAGE and NY-ESO/LAGE-1 within the CD138⁺ cells of patients 4 and 22, respectively. Expression of CGAgs shown as brown stain (M) and CD138 plasma cells are stained with a silver/gray surface stain (P). Images were captured with a Nikon Eclipse E400 microscope with a 40×/0.65 NA objective (Tokyo, Japan) and a Nikon E995 camera.