Characterization of 2L-Rap-hLL1-γ4P. (A) SE-HPLC trace. A single peak was observed at 7.6 minutes, which was shorter than the retention time observed for hLL1 (about 8 minutes). (B) Reducing SDS-PAGE analysis. Lanes 1 and 4: Mr markers; lane 2: 2L-Rap-hLL1-γ4P; lane 3 IgG. Two closely migrating bands attributed to the fused light chains, both larger than the light chain of hLL1, are observed in lane 2.