NES1 region of MALT1 and its role in subcellular localization. (A) Amino acid sequence of the NES1 region in alignment with MALT1 homologs of various species. The amino acid numbers are based on amino acid sequences from accession numbers BAA83099 (Homo sapiens), AAG38590 (Danio rerio), AAG38591 (Caenorhabditis elegans), and AAG38592 (Dictyostelium discoideum) in GenBank. Residues that resemble the consensus sequence of leucine-rich NES are boxed. Alanine substitutions were made at Leu³⁶² and Leu³⁶⁵ for studying its role in subcellular localization (MALT1[1-380]-NES1 mutant). NES1 indicates the NES1 region. (B) Cells transfected with either MALT1(1-380)-NES1 mutant or wild type are shown. The cells were processed in the same manner as those in Figure 2. Relative proportions of cells with specific subcellular localization patterns are shown below each panel. NES1 mutant represents MALT1(1-380)-NES1 mutant and wild-type MALT1(1-380). C indicates cytoplasm; CN, both cytoplasm and nuclei.