Subcellular localization of GFP fusion proteins containing NES regions with or without LMB treatment. (A) Protein structures of various GFP fusion MALT1 deletion mutants; GFP and full-length MALT1 expression vector are shown. NES1 and NES2 indicate the NES1 and NES2 regions, respectively. Numbers below the structures indicate the amino acid number of MALT1, and the subcellular localization pattern is shown on the right of each structure. C indicates cytoplasm; CN, both cytoplasm and nuclei. (B) COS7 cells (2 × 10⁴) were transfected with 0.2 μg of each of the expression vectors. The cells were treated with 4 ng/mL LMB for 18 hours before examination with an immunofluorescence microscope. Nuclei were stained with propidium iodide.