MALT1 deletion mutant structures and their subcellular localization with or without LMB treatment. Protein structures of various deletion mutants are shown. Numbers in the parentheses and below the structures indicate the amino acid number of MALT1. NES1 and NES2 represent the NES1 and NES2 regions (329-380AA and 461-562AA), respectively, while NES1R indicates an NES1 regulatory region (381-420AA). These regions are shown by bars below the constructs. For the nuclear localization study, 2 × 10⁴ COS7 cells were transfected with 0.2 μg of each of the expression vectors. Treatment with 4 ng/mL LMB was conducted 18 hours after transfection, and 21.5 hours after transfection, 25 μM MG132 was added to all of the wells for the visualization of unstable proteins. At 24 hours after transfection, the cells were examined with an immunofluorescence microscope (see Figure 1). Subcellular localization is shown on the right of each structure. C indicates cytoplasm; CN, both cytoplasm and nuclei.