1α,25(OH)₂D₃ inhibits the expression of numerous IFN-γ–induced genes. Real-time quantitative PCR analysis of IFN-γ–induced gene expression. BMDMs isolated from WT or Vdr-KO mice were incubated with IFN-γ or 1α,25(OH)₂D₃ or both. Gene-specific primers were used for amplification and mRNA expression was normalized as described. Analysis was performed in duplicate and data are presented as the mean ± SEM. Differential gene expression was confirmed at least in 2 independent experiments.