GR- and MR-binding assay. Human PRP was incubated with [H³]-dexamethasone or [³H]-aldosterone, and binding assays were performed as detailed in “Materials and methods.” (A) Scatchard analysis of [³H]-dexamethasone binding. (B) Scatchard analysis for [³H]-aldosterone binding. (C) The effect of cold prednisolone (Pred), dexamethasone (Dex), and aldosterone (Aldo) on [³H]-dexamethasone binding to platelets. (D) As in panel C, using [³H]-aldosterone as tracer. V indicates vehicle. Data are mean ± SEM of 2 experiments with different PRP preparation for the Scatchard analyses, and of 4 experiments performed in duplicate for the competition analyses.