Detection of GR in human platelets by flow cytometry and Western blotting analyses. (A) Washed platelets were permeabilized with saponin (“Materials and methods”) and stained with FITC-conjugated anti–P-selectin antibody or PE-conjugated anti-GR antibody. (Ai) Negative primary antibody control; (Aii) positive staining for GR; (Aiii) positive staining for P-selectin; and (Aiv) 2-color staining demonstrating dual positivity of platelets for P-selectin and GR (representative of data from 3 donors). (B) Human washed platelets and correspondent PBMC samples express GR; platelet GR has identical MW to PBMC GR. Blot is with samples from 3 distinct donors, and it is representative of 3 separate experiments.