Detection of GR in human platelets by confocal analysis. Washed platelets were permeabilized with saponin or left intact (“Materials and methods”) and stained with FITC-conjugated anti–P-selectin antibody or phycoerythrin (PE)–conjugated anti-GR antibody. Single-color images are shown, as well as the combined image (far-right panels). Note the large presence of yellow staining (dual labeling) in these 2 panels. Bars, 10 μm.