Serial transplantation of gp130-deficient bone marrow reconstitutes hematopoiesis in irradiated mice. (A) Bone marrow cells recovered from CD45.1⁺ WT mice that underwent transplantation with CD45.2⁺ WT or gp130^flox/flox/TCre cells were transplanted into irradiated CD45.1⁺ WT secondary recipients. After 16 weeks, flow cytometry was used to determine the level of CD45.2 donor-cell contribution to the myeloid, B-lymphoid, and T-lymphoid populations in the reconstituted bone marrow. (B) The c-kit^HiSca-1⁺, c-kit^HiSca-1^-, and c-kit^LoSca-1^-/Lo populations were sorted from the reconstituted bone marrow. Genomic DNA from each population was subjected to PCR analysis (Figure 1A-B) to identify the WT gp130 allele or the excised floxed gp130 allele. DNA was pooled from 4 mice for each genotype. Data are representative of 2 independent experiments.