Generation of gp130^flox/flox/TCre mice. (A) Schematic diagram of portions of the WT gp130 allele, the gp130^flox/flox allele, and the gp130^flox/flox allele after Cre-mediated excision of the floxed exon.⁴  Exon numbers are shown. The loxP sites are depicted as large arrowheads adjacent to the introduced XhoI restriction sites. Small arrowheads indicate the sites of PCR primers that were used to amplify each allele. (B) Genotype analysis. gp130^flox/+/TCre mice were bred with gp130^flox/+ mice. PCR products of genomic DNA from the offspring were resolved on agarose gels before or after digestion with XhoI. Arrows indicate the products corresponding to the WT allele, the floxed allele, and the floxed allele after Cre-mediated excision of the floxed exon.