Further characterization and quantification of coated platelets. Platelets were left unstimulated or activated by the indicated agonists for 7 minutes, stained with (A) FITC-antifibrinogen and PE-JON/A, or (B) FITC-annexin V and PE-JON/A, and analyzed by 2-color flow cytometry. Dashed boxes indicate the unstimulated, activated, and coated platelet populations. (C) Quantification of the coated platelet population following stimulation by various agonists was performed by 2-color flow cytometry with FITC-antifibrinogen and PE-JON/A; n = 5.