Phenotype of the MN1-TEL–positive T-lymphoid tumors. (A) GFP expression in MN1-TEL/Mx1-Cre tumor cells. Solid line indicates tumor cells; broken line, control MN1-TEL/Mx1-Cre thymocytes not treated with pI-pC. (B) MN1-TEL protein in tumor cells. MN1-TEL in tumor thymocytes was detected on a Western blot with a TEL C-terminal antibody. Gapdh detection was used as loading control. Control: uninduced MN1-TEL/Mx1-Cre thymocytes. (C) Immunohistochemical analysis of BM in mice with tumors. (Left column) BM stained with anti-CD3 or anti-TdT antibody. (Right column) Controls without primary antibody. Counterstaining: hematoxylin. (D) Surface marker expression in tumor cells using FCM analysis. (E) Clonality of tumor cells. Tumor DNAs digested with EcoRI were hybridized with a probe for T-cell receptor Jβ2. Control: liver DNA. Arrowhead indicates germ line band. (F) Transplantation of MN1-TEL–positive tumor cells into syngeneic recipient mice. Tumor cells (1 × 106 per recipient, n = 6) were intravenously injected into recipients that were irradiated with 7.5 Gy. A survival curve is shown. (G) M-G staining of tumor cells of the recipient BM. The same type of tumor cells as original tumors were seen in all recipients.
