The CUB-1A peptide coelutes with ULVWF from a gel filtration column. Biotinylated CUB-1A peptide and CUB-1As were incubated with UVLWF for 15 minutes and then allowed to pass through a gel filtration column at a flow rate of 1 mL/min. The eluate was collected in 14 fractions of 1 mL each and probed for the peptide with a polyclonal antibiotin antibody and for ULVWF with a monoclonal VWF antibody. The CUB-1A peptide coeluted with ULVWF (A), whereas the CUB-1As peptide did not (B). The figure is representative of 3 separate experiments.