Effect of DC treatment of LCMV-specific immunity in vivo. Naive BALB/c mice were injected intraperitoneally with 10⁶ of the indicated DC subset on days -10 and -3, followed by LCMV infection (10⁵ pfu) on day 0. The LCMV-specific T-cell response was analyzed by restimulation of splenocytes in vitro with 10 μg/mL NP-118 peptide for 4 hours and analyzed by ICCS as described. (A) Representative FACS diagrams of the numbers shown in panel B. (B) Total number of NP-118 reactive IFN-γ⁺CD8⁺ splenocytes after ICCS. (C) Representative FACS diagrams of CD8 expression as shown in panel D. (D) Quantification of the percent CD8^lo and CD8^hi spleen cells. In panels B and D, each dot represents an individual mouse. ^**P < .01; ^***P < .005 compared with PBS group.