T-cell activation in vivo by DC subsets. BALB/c mice were treated with 10⁶ syngeneic DCs on days -14 and -7. Splenocytes were harvested on day 0 and 10⁶ cells were cultured in medium containing 10% FBS (top) or 1.5% NMS (bottom) for 72 hours and cytokine production was analyzed. Each dot represents an individual mouse.