Levels of NF-κB activity as determined by EMSA for various FAB subtypes compared with marrow from healthy donors. Shown are results with nuclear extracts from bone marrow mononuclear cells (ie, the same population on which flow analysis was carried out). NF-κB activity was determined by densitometry using ImageQuant software. Results represent intensity ratios of patient samples over healthy controls against normal. The horizontal axis represents flow scores which were calculated as described in Table 1.³⁴  The regression line illustrates correlations of flow scores and NF-κB activity (r = 0.55; 95% CI, 0.36 to 0.74; P = .01).