Oxidized β₂-GPI induced release of cytokines by DCs. Five-day human DCs were stimulated with or without ox-β₂-GPI (10 μg/mL), H₂O₂-β₂-GPI (10 μg/mL), and LPS (100 ng/mL). Supernatants were collected after 48 hours to measure IL-1β, IL-8, IL-12p70, IL-6, TNF-α, and IL-10 by specific ELISA experiments. Ox-β₂-GPI and H₂O₂-β₂-GPI triggered statistically significant up-regulation of all cytokine secretion (P < .001). Results are expressed as mean value ± SD of 11 independent experiments.