Separation of γ-carboxylated and uncarboxylated FX by hydroxyapatite chromatography. Separation of the γ-carboxylated and uncarboxylated factor X is as described in “Materials and methods” and as previously described.¹⁴  Cell line abbreviations are as defined in Figure 1. In each panel the first peak represents the uncarboxylated factor X and the second peak is fully γ-carboxylated factor X. (A) HEK293-FX (A6). (B) HEK293-FX (A6)–VKOR. (C) HEK293-FX (A6)–GGCX. (D) HEK293-FX (A6)–VKOR-GGCX. Diagonal lines indicate sodium phosphate concentration gradient of elution.