The p130Cas is not required for NPM-ALK–mediated migration. (A) Cas^-/- and Cas^+/+ rescued fibroblasts were infected with Pallino NPM-ALK retrovirus and then sorted for GFP content. Protein expression levels were verified by WB as indicated. (B) Cells were plated on a Matrigel-coated insert and the number of migrated cells was evaluated after 48 hours. The histograms represent the average number of migrated cells from 3 independent experiments using triplicate wells for experimental point. (C) CEM lymphoblastoid cells were infected with Pallino retroviruses containing NPM-ALK or NPM-ALK^K210R together with retrovirus for p130Cas. Protein expression levels were verified by WB as indicated. (D) The histograms represent the average numbers of migrated cells in response to increasing concentrations of SDF-1α as indicated. Data are from 1 of 2 independent experiments. Error bars indicate standard deviation. ^*Statistically significant analysis as measured by the Student t test.