Role of ST3Gal-IV in selectin ligand formation in Th1 cells. Rolling of WT and ST3Gal-IV^-/- Th0 and Th1 cells on (A) E-selectin and (B) P-selectin reveals significantly decreased rolling of ST3Gal-IV^-/- Th1 cells, compared with WT Th1 cells. (C) qRT-PCR shows no significant difference in ST3Gal-IV^-/- mRNA levels between WT or T-bet^-/- Th0 or Th1 cells. (D) qRT-PCR also shows no significant difference in ST3Gal-IV mRNA levels between WT and Stat4^-/- Th0 or Th1 cells. Data are depicted as in Figure 4A. Results are representative of at least 3 experiments. ^**Significantly different from all other groups.