Figure 2.
Proteomics analysis of TSP-1 production by DCs in response to ATPγS. (A) DCs were either untreated (Ctrl) or treated with ATPγS (100 μM) or PGE2 (500 nM) for different periods of time in complete medium. Supernatants were collected at the end of incubation to perform Western blotting using an anti–TSP-1 rabbit polyclonal antibody (1:200). (B) DCs were treated with ATPγS (100 μM) for 4, 8, or 24 hours in serum-free medium. At the end of incubation, supernatants were collected and proteins were extracted and loaded on an 8% SDS–polyacrylamide gel. After colloidal Coomassie blue staining, proteins of interest (black arrows A and B) were excised and in-gel digested by trypsin.
