Production of TSP-1 by human monocyte–derived DCs in response to nucleotides. (A) DCs were stimulated for various periods of time with either ATPγS (100 μM), PGE₂ (500 nM), or LPS (100 ng/mL). (B) DCs were challenged with various concentrations of ATPγS (•) or PGE₂ (○) for 24 hours. (C) DCs were challenged with various concentrations of ATPγS (•) or ATP (○) for 24 hours. (D) DCs were stimulated for 24 hours with ATPγS, AR-C67085MX, BzATP, and ADPβS at 100 μM and with ATP, ADP, UTP, UDP, and UDP-glucose at 300 μM. Supernatants of treated DCs were harvested for ELISA measurements of human TSP-1. Results are expressed as nanograms per 10⁶ cells/mL and represent the mean ± range of duplicate points in 1 representative out of 3 (A) or 2 (B-D) independent experiments.