Troglitazone down-regulates the expression of nuclear localized c-Rel. Peripheral blood adhering monocytes were cultured with GM-CSF and IL-4 with or without troglitazone from the first day of culture. For activation of DCs, cells were incubated with different toll-like receptor ligands 24 hours before preparing nuclear extracts. Nuclear localized c-Rel protein was detected by SDS-PAGE and Western blot. Ponceau S staining was performed to ensure equal loading of the gel.