ΔHb variation and linkages with data of donors and patients with TDT. (A) ΔHb variation (normalized values per RBC unit) among patients with TDT shortly (post) and 1-week (week) after transfusion. The cohort medians (grams per deciliter) that distinguish low from high transfusion response events are denoted by arrows. (B-C) Network presentation of RBC unit variables, donor variables, and baseline variables of patients with TDT significantly correlated (P < .05) with ΔHbpost (B) and ΔHbweek (C) according to the univariate analyses shown in supplemental Table 4 and supplemental Table 6, respectively. Edge length is inversely proportional to the correlation coefficient (r), with shorter edges indicating stronger correlations. Black/solid lines, positive correlations; red/dashed lines, negative correlations. Metabolite (m) numbers in nodes correspond to definitions in supplemental Tables 4 and 6. AtrF, atrial fibrillation; CFH, cell-free Hb; DHb, ΔHb; EXMH, extramedullary hematopoiesis; HCT, hematocrit; Hct, hematocrit; LDH, lactate dehydrogenase; m, membrane; MCF, mean corpuscular fragility; MON, monocyte; MUT, mutation severity; PH, pulmonary hypertension; Phe, phenylalanine; PPA, proteolytic proteasome activity; PPA(U), PPA of RBC units; PPAm(U), proteolytic proteasome activity at the RBC membrane (units); PSRBC(U), PS-exposing RBCs (units); RDW, red cell distribution width; RET, reticulocytes; sTFR, soluble transferrin receptor; TBV, total blood volume; 1TRF, age of transfusion initiation; TRFInt, transfusion interval; TRV, total RBC volume; UAdAC, UA-dependent antioxidant capacity; UAdAC(U), UAdAC of RBC units; UrocA(U), Urocanic acid (units) ; VOL(U), volume of RBC units.