Variation in plasma metabolites across transfusion time. (A) Heat map showing the mean differential expression of the top 60 metabolites identified by targeted metabolomics across time points. (B) Summary of TDT metabolites that changed significantly (fold change vs before; P < .05) in the posttransfusion period (Post, Week), based on ANOVA with post hoc tests. Fold change levels (vs before [Pre]) in RBC unit supernatants are also presented. Red tones, increase; blue tone, decrease. The metabolites are classified in 3 groups based on their response to transfusion: those showing reversible early response, irreversible early response, or late response. Representative examples are shown in the box plots (C-E), denoting the normalized (cube root transformation) and scaled (autoscaling) peak areas of metabolites with pronounced differences between the 3 timepoints. The bars of the box plots depict the median along with the interquartile range. ∗P < .05 vs before; #P < .05 vs after. 2-ABA, 2-amino-isobutyrate; GABA, γ-aminobutyrate; MCiOP, mono-(carboxy-iso-octyl) phthalate; MiNP, monoisononyl phthalate.