Fetal ME induction causes myeloid-biased gene expression without altering chromatin accessibility. (A) UMAP representing control and ME-expressing P28 LK cells based on scATAC-seq profiles. Clustering was performed in ArchR. (B) Cluster assignments based on inferred gene expression. The assignments follow the clustering nomenclature specified in Figure 2A. (C) Distribution of cells from fetal and postnatal ME induction groups within scATAC-seq clusters. (D) Inferred CITE-seq identities for cells within scATAC-seq clusters 3 and 6. (E) Myeloid identity scores, based on quadratic programming using integrated CITE-seq data, for cells within the indicated scATAC-seq clusters after fetal or postnatal ME induction. (F) Pseudotime analysis of transcription factors with concordant differential expression (based on integrated CITE-seq data) and motif accessibility (based on scATAC-seq) through the course of myeloid differentiation, after fetal or postnatal ME induction. Genes in red were identified in both cohorts. The trajectory is shown above the heat map. (G) Sox4, Mef2c, and Mybl2 transcript expression in individual cells, projected as heat maps on the UMAPs from panel A. Ranges of min/max expression are identical for fetal and postnatal induction cohorts for each gene. (H) Enrichment for SOX4, MEF2C, and MYBL2 motifs within individual cells, projected as heat maps. min, minimum; max, maximum; UMAP, uniform manifold approximation and projection.