Fetal MLL::ENL (ME) induction conveys heritable protection against leukemogenesis. (A) Summary of experimental design for ME induction and transplantation experiments. (B) Kaplan-Meier survival curves for recipient mice transplanted with 300 000 P0 liver cells or 500 LSK cells after ME induction at E10.5 or P14. (C) Donor (CD45.2) leukocyte engraftment in surviving recipient mice at 8 months after transplantation. (D) Summary of experimental design for panels E-F and for CITE-seq experiments in Figure 2. (E) Kaplan-Meier survival curves and 16-week donor chimerism for recipient mice after ME induction at E10.5 or P0, and transplantation at P28. (F) ME transgene expression (CPM) based on RNA sequencing. n = 4 biological replicates per group, ∗∗∗P < .001 by 1-way analysis of variance with Tukey’s post hoc test. For all survival curves, group sizes and P values are shown in the panels. P values were calculated by the log-rank test. CPM, counts per million; DOX, doxycycline; txp, transplant.