Chord diagram shows the cross-linking profile of fibrin clots. (A) Clots formed from healthy controls. Schematic representation of the FGG (red), FGB (orange), and FGA Q sites (yellow to green) found in cross-links (starting at the top of the circle and moving clockwise). Schematic representation of the FGG and FGA N- to C-terminal K sites (light blue to pink) (from the bottom clockwise to top). The length of the outer arc (forming the circle) is based on the maximum number of cross-link identifications across the patient samples scaled to the maximum number across patients with trauma and controls. The chord shows connectivity of Q sites (right half) to K sites (left half), and widths are based on the number of cross-links. The inner arc matches the color of the corresponding site of connectivity. (B) Clots formed from banked trauma plasma. (C) Structure of fibrinogen (PDB 3GHG) with the αC, β N-termini, and γA C-termini regions modeled. Relative position of cross-linked lysine (K) domains (matching panels A-B) shown for the 3 chains on the left half of fibrinogen and glutamine (Q) domains on the right half.