Impact of miR-223-3p modulation on platelet procoagulant activity. (A) Relative fold increase in the proportion of procoagulant platelets after activation with thrombin and convulxin. (B) Quantification of platelet-supported thrombin generation (velocity index parameter) after activation with TRAP and collagen. miR-223-3p upregulation relative to the negative control (left); miR-223-3p downregulation relative to the negative control (right). (C) Calcium flux (Fluo-3-AM MFI) quantification over time, before and after activation with thrombin and convulxin. Data are from 4 to 9 independent experiments. ∗P < .05; ∗∗P < .01; ∗∗∗P < .001. Fluo-3-AM, fluo-3-pentaacetoxymethyl ester; MFI, mean florescence intensity; ns, non-significant.