Metabolism changes from pretransfuion to posttransfusion in murine platelets. (A) Visual outline of the study. (B) Heat maps of metabolites (mean-centered and divided by the standard deviation of each variable) of platelet-rich plasma (CSP, left panel; RTP, right panel) after storage (pretransfusion, left) and after transfusion into thrombocytopenic animals (posttransfusion, right). Metabolite groups are color-coded to correspond with volcano plots in (C-D). (C-D) Volcano plots of CSP (C) and RTP (D), with the colors of the dots corresponding to the metabolite groups in the heat maps. Dots above the dashed line are below the FDR significance cutoff of q = 0.05, following the Benjamini-Hochberg procedure for P values derived from a 2-tailed, unpaired Student t test. Principal component analyses for CSPs (E) and RTPs (F). (G) Heat maps of the top 25 clustered metabolites in CSP with posttransfusion values averaged between recipients of the same donor. (H) Heat maps of the top 25 clustered metabolites in RTP with posttransfusion values averaged between recipients of the same donor. AS, amino sugars; FDR, false discovery rate; GPL, glycerophospholipid synthesis; LC-MS/MS, liquid chromatography–tandem mass spectrometry; PPP, pentose phosphate pathway; TCA, tricarboxylic acid cycle.

Metabolism changes from pretransfuion to posttransfusion in murine platelets. (A) Visual outline of the study. (B) Heat maps of metabolites (mean-centered and divided by the standard deviation of each variable) of platelet-rich plasma (CSP, left panel; RTP, right panel) after storage (pretransfusion, left) and after transfusion into thrombocytopenic animals (posttransfusion, right). Metabolite groups are color-coded to correspond with volcano plots in (C-D). (C-D) Volcano plots of CSP (C) and RTP (D), with the colors of the dots corresponding to the metabolite groups in the heat maps. Dots above the dashed line are below the FDR significance cutoff of q = 0.05, following the Benjamini-Hochberg procedure for P values derived from a 2-tailed, unpaired Student t test. Principal component analyses for CSPs (E) and RTPs (F). (G) Heat maps of the top 25 clustered metabolites in CSP with posttransfusion values averaged between recipients of the same donor. (H) Heat maps of the top 25 clustered metabolites in RTP with posttransfusion values averaged between recipients of the same donor. AS, amino sugars; FDR, false discovery rate; GPL, glycerophospholipid synthesis; LC-MS/MS, liquid chromatography–tandem mass spectrometry; PPP, pentose phosphate pathway; TCA, tricarboxylic acid cycle.

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