Schematic diagram of study. The 144-gene nCounter panel was processed on 360 samples: 195 from FFPE tissues qualified by on-site expert hematopathologist review, and 165 from previously extracted RNA with accompanying pathology data, after which 24 samples were removed for inadequate RNA. After iterative modeling, during which 71 duplicate samples were identified and excluded and 78 genes were eliminated, the locked 45-gene predictor was independently validated on 232 unique samples that passed quality control metrics. LLMPP, Lymphoma/Leukemia Molecular Profiling Project; MER, Molecular Epidemiological Resource; QC, quality control.