Comparison of protein domains and amino acid alignment of canonical TNS1 and eTNS1. (A) Comparison of canonical TNS1 (TNS1-201) protein domains with eTNS1 (TNS1-208). The amino acids in pink are unique to eTNS1, whereas the 21 amino acids in gray (1000-1020) in canonical TNS1 are missing in eTNS1. The primary antibody epitope is indicated (amino acids 1326-1339 in TNS1). Figure created with BioRender.com. (Diaz, D. 2025, https://app.biorender.com/illustrations/660ab6fdc5f830fa937a2f31). (B) Amino acid alignment using UniProt align tool showing amino acids coded for by exon 1E in eTNS1 protein, highlighted in pink. Asterisks, identical residues; colon, residues with strongly similar properties (scoring >0.5 in Gonnet PAM 250 matrix); period, residues with weakly similar properties (scoring <0.5 in Gonnet PAM 250 matrix). (C) Cell lysates from control erythroblasts (day 11) were subjected to immunoprecipitation using an anti-TNS1 antibody. Both the immunoprecipitated material and total cell lysate (input) were analyzed by western blot using antibodies against TNS1 and actin. Schematic in panel A created with BioRender.com.