Database mining of ABPs and actin NFs reveals increased TNS1 expression during human terminal erythroid differentiation. (A, top) Schematic of F-actin reorganization (red) into the enucleosome, GPA sorting to the reticulocyte (green), and nuclear expulsion (blue) during human erythroblast enucleation. Figure created with give the Biorender.com. (Diaz, D. 2025, https://app.biorender.com/illustrations/64b97cb2f83e095a50810cbb) (A, middle) Maximum intensity projection of Airyscan Z-stacks of human CD34⁺ cells before and during enucleation stained for GPA (green), F-actin (phalloidin; red), and nuclei (Hoechst; blue). (A, bottom) F-actin staining in grayscale shows the formation of the enucleosome at the rear of the nucleus. Scale bar, 5 μm. (B) Flowchart representing the data-mining strategy for 135 ABP and actin NFs to identify mRNAs and proteins that are upregulated during terminal erythroid differentiation of CD34⁺ cells. Graphs showing fold-change in expression of (C) 97 RNA-seq and (D) 49 proteomics hits during terminal erythroid differentiation. Values were plotted on a linear scale (converted from log₂-fold change) and normalized to their respective expression level at the proerythroblast stage.