Transcriptomic analysis of Asct2 Bcma-CAR T cells and Bcma-CAR T cells before and after BCMA antigen stimulation. (A) Expression of Slc1a5 (Asct2). (B) Number of differentially expressed genes between Asct2 Bcma-CAR T cells and Bcma-CAR T cells before and after BCMA antigen stimulation. (C) PC analysis of gene expression profiles. (D) Gene signatures upregulated in Asct2 Bcma-CAR T cells compared with Bcma-CAR T cells, before and after BCMA antigen stimulation. (E) Heat map showing differential expression of SLC family members in Asct2 Bcma-CAR T cells compared with Bcma-CAR T cells after antigen stimulation. Data represent mean ± SEM, and were analyzed using Student t test (∗∗P < .01). Exp, expression; NES, normalized enriched score; PC, principal component; unstim, unstimulated.