CD44 is neither sufficient nor required for the expression of AnWj antigen, and CD44 expression is not affected by lack of Mal protein. (A) Flow cytometry with anti-Mal (ab167374), anti-CD44 (BRIC222), and anti-AnWj (H86) on wild-type BEL-A cells (top row), CD44 KO BEL-A cells (second row), and CD44 KO BEL-A cells overexpressing either Mal or CD44. Wild-type BEL-A cells express CD44, which is abolished in the CD44 KO BEL-A cells. Overexpression of Mal in CD44 KO BEL-A cells results in detectable expression of Mal and AnWj antigen, which is not observed in either wild-type, CD44 KO, or CD44 KO BEL-A cells rescued by overexpression of CD44. (B) Flow cytometry with anti-Mal (ab167374), anti-CD44 (BRIC222), and anti-AnWj (H86) on wild-type BEL-A cells (top row), Mal KO BEL-A cells (second row), and BEL-A cells overexpressing Mal (row 3). CD44 expression is not altered by either KO or OE of Mal protein. OE, overexpression.