Changes in protein abundance associated with aging. (A) Volcano plot of significantly changed proteins (permuted P < .05) during aging in AML, which were calculated using the Spearman correlation approach. (B-C) Pathway enrichment analysis of proteins showing positive (B) and negative (C) correlation with age through GO, biological process, KEGG, and reactome. (D) Heat maps displaying the trajectories of 10 protein clusters (upper panel) and all proteins (lower panel) that change with age in AML. (E) The 1104 proteins in cluster 2 display a monotonically increasing trend with age. (F) Enriched GO pathways of proteins in cluster 2. The size of the circles represents the count of proteins enriched in the GO term. The thickness of the edges denotes the semantic similarity between each pair of GO terms. The color of the circles indicates the adjusted significance of enrichment.