Biomarkers of RBC hydration correlate with metabolites in PIEZO1-HX. (A-C) Correlations between O_hyper, the main biomarker to represent RBC hydration, and ATP:2,3-DPG ratio (A), PK:HK ratio (B), and PK thermostability (C). (D) Correlation between l-carnitine and PK:HK ratio. (E) Correlation between l-acetylcarnitine and PK thermostability. Phosphoenolpyruvate concentration of 5 mM for both PK:HK ratio and PK thermostability. Square symbols indicate splenectomized patients.