Routine hematologic parameters, ferritin levels, and stomatocyte counts of patients with HX with a PIEZO1 or KCNN4 mutation. (A-D) Various routine hematologic parameters were measured in HCs and patients with PIEZO1- and KCNN4-HX, including hemoglobin level (A), reticulocyte count (B), MCV (C), and MCHC (D). (E-F) Considering that dehydration is characteristic of HX, hypochromic cells (E) and hyperchromic cells (F) were measured. (G) The percentage of stomatocytes (PIEZO1, n = 10) was evaluated. (H-I) In addition, ferritin levels (H) and TSAT percentages (I) were compared between groups (HCs, n = 5). (J-K) Representative peripheral blood smears from PIEZO1-HX whole blood (J) and KCNN4-HX whole blood (K) are shown. Error bars represent median with IQR. ∗∗∗P < .001; ∗∗P < .01; ∗P < .05. Square symbols indicate splenectomized patients. MCV, mean corpuscular volume; TSAT, transferrin saturation.