CD36 association with FcRγ is independent of GPVI in platelets. (A) CD36 was immunoprecipitated from washed human platelets (7 × 10⁸/mL) and immunoblotted for GPVI, CD36, and FcRγ. (i) Representative blots, (ii) densitometric analysis of FcRγ, and (iii) percentage of total FcRγ associated with immunoprecipitated CD36, from 5 independent experiments. (iv) As in subpanel i, except platelets were untreated or treated with oxLDL (50 μg/mL) or nLDL (50 μg/mL) for 15 seconds. Representative blots from at least 3 independent experiments. (B) Washed platelets (5 × 10⁸/mL) from WT, CD36^−/−, and GPVI^−/− mice were either untreated or treated with oxLDL (50 μg/mL) or CNX (500 ng/mL), lysed, and immunoblotted for phospho-tyrosine. Membranes were stripped and reprobed with GAPDH antibody. (i) Representative blots and (ii) densitometry analysis for phospho-Syk from 3 independent experiments. ∗∗P < .01. CNX, convulxin; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; IgG, immunoglobulin G; WCL, whole-cell lysate.