Figure 1.
Carbon labeling of glycolytic metabolites in patients with HK deficiency. Purified RBCs from 10 controls and 2 patients (HKD1-2) with HK deficiency were incubated with 13C6-glucose. The incorporation of the labeled carbon atoms in downstream glycolytic metabolites was determined over time by quantifying the concentrations of carbon-labeled metabolites with LC-HRMS. Lactate concentrations were measured both intracellularly and in the medium. Data points with error bars indicate mean and standard deviation.
