Analyzing BCMA-specific tetramer⁺ CD8⁺ T cells and infiltration of T cells in splenic tissue. C57BL/6J mice received different treatment groups by either IV or IM injection. Five days after a single IV dose, and 7 days after the first IM dose in a schedule of 2 doses separated by 3 days, the mice were euthanized, and their splenocytes were stained to assess the BCMA-specific tetramer⁺ CD8⁺ T cells by flow cytometry. (A-B) Percentage of BCMA-specific tetramer–positive cells analyzed on splenocytes after IV and IM injections in different treatment groups. (C) The spleens were further analyzed for infiltrating CD3⁺ and CD8⁺ T cells by immunofluorescence staining. The representative images demonstrate the infiltrating CD3⁺ (green) and CD8⁺ (yellow) T cells in splenic tissue. (D-E) The percentage of CD8⁺ T cells infiltrating into the spleen after different treatments is shown after IV and IM injection. The data are shown as mean ± SD from at least 3 independent biological experiments. Statistical analysis was performed using an unpaired Student t test: ∗P < .05. DAPI, 4′,6-diamidino-2-phenylindole.