Transfection and translation efficacy of mRNA LNPs in MoDCs. (A-D) MoDCs were treated with free Cy5-tagged BCMA mRNA, Cy5-tagged BCMA mRNA packaged in LNPs, or Cy5-BCMA-mRNA LNPs codelivered with poly(I:C) LNPs. They were evaluated for cellular uptake of Cy5-tagged BCMA-mRNA LNPs using flow cytometry after 1 hour and 2 hours of incubation, and cytosolic localization (red signal) was evaluated by confocal microscopy after 1 hour. Quantification was performed for respective treatment groups using flow cytometry and confocal microscopy. (E-H) MoDCs were treated with free GFP mRNA, GFP-encoding mRNA packaged in LNPs, or GFP-encoding mRNA LNPs codelivered with poly(I:C) LNPs. MoDC uptake of GFP was analyzed by flow cytometry after 12 hours and 24 hours of incubation, and cytosolic expression was evaluated by confocal microscopy after 24 hours of incubation. GFP quantification was determined for respective treatment groups using flow cytometry and confocal microscopy. The data are shown as mean ± standard deviation (SD) from at least 3 independent biological experiments. Statistical analysis was performed using an unpaired Student t test: ∗P < .05. DAPI, 4′,6-diamidino-2-phenylindole.