Figure 1.
Synthesis and characterization of mRNA LNPs. (A) The different lipid components used for the packaging of BCMA mRNA and poly(I:C) in LNPs. (B-C) The cryogenic electron microscopy images of the BCMA-mRNA LNPs and poly(I:C) LNPs. (D-E) Particle size analysis performed for BCMA LNPs (82.3 ± 6.75 nm) and poly(I:C) LNPs (117.6 ± 5.86 nm) by dynamic light scattering. (F) The loading percentage of BCMA-mRNA LNPs and poly(I:C) LNPs. (G) The gel electrophoresis shows the stability of free mRNA and mRNA packaged in LNPs after incubation in human serum for 2 hours at 37°C. (H) The switch in surface charge from negative at physiological pH 7.4 to positive at pH 5, mimicking the acidic endosomal environment, confirmed the efficient endosomal escape of the mRNA into cytosol. DOPE, 1-2-dioleoyl-sn-glycero-3-phosphoethanolamine.

Synthesis and characterization of mRNA LNPs. (A) The different lipid components used for the packaging of BCMA mRNA and poly(I:C) in LNPs. (B-C) The cryogenic electron microscopy images of the BCMA-mRNA LNPs and poly(I:C) LNPs. (D-E) Particle size analysis performed for BCMA LNPs (82.3 ± 6.75 nm) and poly(I:C) LNPs (117.6 ± 5.86 nm) by dynamic light scattering. (F) The loading percentage of BCMA-mRNA LNPs and poly(I:C) LNPs. (G) The gel electrophoresis shows the stability of free mRNA and mRNA packaged in LNPs after incubation in human serum for 2 hours at 37°C. (H) The switch in surface charge from negative at physiological pH 7.4 to positive at pH 5, mimicking the acidic endosomal environment, confirmed the efficient endosomal escape of the mRNA into cytosol. DOPE, 1-2-dioleoyl-sn-glycero-3-phosphoethanolamine.

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