The transcriptional states of the treated mice. (A) Two-dimensional UMAP of all cells. A total of 12 773 cells passed quality control, capturing 12 populations, depicted with distinct colors, corresponding to 4 major lineages, including T cells, B cells, plasma cells, and myeloid cells. (B) UMAP of the T-cell compartment; 10 T-cell populations were identified and are depicted with distinct colors. (C) Heat map capturing the expression of marker genes across the distinct cell populations. (D-E) Box plots portraying cell proportion differences among the mice treated with uTcs + IgG (n = 8), uTcs + SAR (n = 6), veTcs + IgG (n = 8), veTcs + SAR (n = 8). (D) CD8 and CD4 total T cells and (E) specific T-cell subsets, including CD4 T helper cells and CD4 and CD8 cytotoxic T-cell populations. Significantly different proportions among pairwise comparisons are denoted with asterisks (∗FDR < 0.05, ∗∗FDR < 0.01; Dirichlet regression, Benjamini-Hochberg FDR correction). NKT, natural killer T cells; Tcm, T central memory; Temra, T effector memory RA; UMAP, uniform manifold approximation and projection.