Treatment with TQ depleted IL-1Rα, IL-8/CXCL8, MIP-1β, and other cytokine expressions in PD post-MPN sAML cells. PD post-MPN (mutant CALR) sAML cells (sample 16 on the oncoplot) were treated in duplicate with the indicated concentrations of TQ for 48 hours. At the end of treatment, culture supernatant was harvested and frozen at −80°C. A 45-cytokine panel assay was used to determine the levels of cytokines including IL-1Rα, IL-8/CXCL8, MIP-1β, SDF1α, HGF, LIF, and CXCL1 in the untreated and TQ-treated samples. ∗P < .05; ∗∗P < .01; ∗∗∗P < .005; ∗∗∗∗P < .001.