Treatment with TQ induced concordant alterations in the transcriptome and proteome of post-MPN sAML cells and reduced protein expression of S100A8 and S100A9 in phenotypically defined post-MPN sAML stem/progenitor cells. (A) PD (JAK2-V617F) sAML cells (sample 13 on the oncoplot) were treated with 20 μM of TQ for 16 hours in biologic duplicates. After this, total RNA was isolated and used for RNA-seq analysis. The volcano plot shows the log₂ fold-change vs –log₁₀P value for all mRNA with >1.25-fold change up or down with a P value <.05. (B) PD (JAK2-V617F) sAML cells (sample 13 on the oncoplot) were treated with the indicated concentration of TQ for 48 hours in biologic duplicates. At the end of treatment, cells were harvested and used for whole-proteome tandem mass spectrometry. The volcano plot shows the log₂ fold-change vs –log₁₀P value for all proteins with >1.2-fold change up or down with a P value <.05. (C) Log₂ fold-change in selected protein expressions in TQ-treated PD post-MPN sAML cells compared with dimethyl sulfoxide control cells. Threshold is proteins with greater than a 1.2-fold change up or down and a P value <.05. (D) Scatterplot of TQ-induced, concordant mRNA and protein expression changes in PD post-MPN sAML 13 cells. (E) PD (JAK2-V617F) sAML cells (9 and 14 from the oncoplot) were treated with 20 μM of TQ for 48 hours. Cells were used for CyTOF analysis with a cocktail of rare metal–tagged antibodies to define stem/progenitor cells and other sAML-relevant oncoproteins. Panel shows a heat map of log₂ fold-change in protein expression from cells treated with 20 μM of TQ for 48 hours compared with the control cells. Hi, high; Lo, low.