Treatment with TQ depletes mRNA expression and causes negative enrichment of MYC targets, inflammatory response and IL-6–JAK/STAT signaling pathways in post-MPN sAML cells. (A) PD post-MPN (JAK2-V617F) sAML cells (number 6 on the oncoplot) were treated with 20 μM of TQ for 16 hours as biologic replicates. Total RNA was isolated and used for RNA-seq analysis. The heat map shows the number of mRNAs depleted or induced >1.25-fold with a P value <.05. (B) Gene set enrichment analysis of TQ-treated sAML cells compared with HALLMARK and REACTOME pathways. All q-values are <0.1. (C-D) Gene set enrichment plots for HALLMARK_MYC_TARGETS, INFLAMMATORY RESPONSE, and IL-6_JAK_STAT3_SIGNALING. (E) Volcano plot of log₂ fold-change vs –log₁₀P value for all mRNA with >1.25-fold change up or down and a P value <.05. (F) Log₂ fold-change (P < .05) for TLR4 signaling genes in the RNA-seq analysis from the PD sAML cells (biologic replicates) treated with 20 μM of TQ for 16 hours. FDR, false discovery rate; NES, normalized enrichment score.